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Clinical Chemistry 22: 1589-1592, 1976;
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Clinical Chemistry, Vol 22, 1589-1592, Copyright © 1976 by American Association for Clinical Chemistry

Automated method for L-carnitine determination

DW Seccombe, P Dodek, J Frohlich, P Hahn, JP Skala and DJ Campbell

Because of renewed interest in a possible connection between carnitine, lipid disorders, and myopathy, an automated method of analysis is desirable. Deproteinization of serum by use of membrane filter cones and automated assay with a bichromatic analyzer (the ABA-100) substantially increases efficiency without sacrificing the specificity and accuracy of the original manual enzymatic method. The described procedure allows for analysis of 80 speciments a day and is thus suitable for screening of selected populations. Normal values found in blood sera of adults were in the range of 25.0-73.8 mu mol/liter and the method has sufficient sensitivity to accurately measure concentrations as small as 10 mu mol/liter.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
L. Wan and R. W. Hubbard
Determination of free and total carnitine with a random-access chemistry analyzer
Clin. Chem., April 1, 1998; 44(4): 810 - 816.
[Abstract] [Full Text] [PDF]


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