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Clinical Chemistry 22: 1593-1595, 1976;
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Clinical Chemistry, Vol 22, 1593-1595, Copyright © 1976 by American Association for Clinical Chemistry

Determination of serum retinol (vitamin A) by high-speed liquid chromatography

MG De Ruyter and AP De Leenheer

We report a fast and simple high-speed liquid-chromatographic assay for serum retinol. Only 100 mul of serum is required. The lower detection limit is 50 mug/liter; linearity was demonstrated up to 1.50 mg/liter. On analyzing a serum pool eight times, a CV of 2.5% was obtained. Values by this method are compared with results obtained by a flurometric method [Clin. Chem. 16, 766(1970)].


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Copyright © 1976 by the American Association for Clinical Chemistry.