Clinical Chemistry
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Clinical Chemistry 22: 1614-1617, 1976;
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Clinical Chemistry, Vol 22, 1614-1617, Copyright © 1976 by American Association for Clinical Chemistry

Direct enzymatic determination of urea in plasma and urine with a centrifugal analyzer

JP Bretaudiere, HT Phung and M Bailly

A direct enzymatic micromethod (sample volume, 3mul) has been adapted to the centrifugal analyzer (ENI-GEMSAEC) for measurement of urea in plasma and urine. The method is based on urease (urea amidohydrolase, EC3.5.1.5)/glutamate dehydrogenase [l-glutamate:NAD(P)+oxidoreductase (deaminating), EC1.41.3] coupled reactions, and uses a two-point fixed- time (t(1)=20s,t(2)=50s)kinetic scheme for monitoring the rate of comsumption of NADH at 340 nm. Sensitivity and precision of the method are excellent,and results compare well with those from a commonly used continuous-flow method.




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Copyright © 1976 by the American Association for Clinical Chemistry.