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Clinical Chemistry 22: 1655-1658, 1976;
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Clinical Chemistry, Vol 22, 1655-1658, Copyright © 1976 by American Association for Clinical Chemistry

Automated radioimmunoassay of choriomammotropin (human placental lactogen)

A Castro, I Prieto, C Wunsch, G Ertingshausen and H Malkus

We describe a totally automated procedure for radioimmunoassay of choriomammotropin, in which all phases of the assay are automated in a single system ("Centria"). This system permits the simultaneous incubation and separation of many samples in a nonequilibrium assay, and measurements are obtained in less than 30 min. Results for clinical samples by reference radioimmunossay methodology and with the Centria system compared uniformly well: y=0.91x - 0.87;r=0.94. The coefficient of variation for samples run in duplicate on the same day was 5.2%, 7.4% for samples run on different days. The specificity, sensitivity, simplicity, and speed of this system makes it a useful new tool for kinetic, nonequilibrium immunoassay.




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Copyright © 1976 by the American Association for Clinical Chemistry.