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Clinical Chemistry 22: 1692-1696, 1976;
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Clinical Chemistry, Vol 22, 1692-1696, Copyright © 1976 by American Association for Clinical Chemistry

Results for serum cholesterol and triglycerides by gas-liquid chromatography, as compared with a continuous-flow technique

R Watts, T Carter and S Taylor

We assessed the usefulness of gas-liquid chromatography forthe routine and reference measurement of cholesterol and triglycerides (triacylglycerols). Compared with results obtained by an AutoAnalyzer II method, correlation was good with both serum specimens (triglycerides, r = 0.88; cholesterol, r = 0.90) and lipoprotein fractions isolated by ultracentrifugation (triglycerides, r = 0.98; cholesterol, r = 0.98). However, none of the comparisons was completely free of analytical bias, and in general the AutoAnalyzer method underestimated the cholesterol value and overestimated triglycerides. The obvious advantage of the AutoAnalyzer is its greater analytical speed, but the chromatographic method appeared to be more accurate and precise and to suffer less from interfering substances, and in addition gave information about the cholesterol ester and triglyceride composition. We expect gas-liquid chromatography to be of major importance in the calibration of other analytical methods.




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Copyright © 1976 by the American Association for Clinical Chemistry.