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Clinical Chemistry 22: 1702-1704, 1976;
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Clinical Chemistry, Vol 22, 1702-1704, Copyright © 1976 by American Association for Clinical Chemistry

Enzymatic, fluorometric assay of alpha-amylase in serum

GG Guilbault and EB Rietz

An enzymatic, fluorometric method for kinetic assay of serum alpha- amylase (1,4alpha-D-glucan 4-glucanohydrolase, EC 3.2.1.1) is described. A soluble starch is used as substrate, in tris(hydroxymethyl)methylamine buffer. All measurements are made in Pyrex cuvettes at 37 degrees C, with a reaction volume of 1.16 ml. The assay is based on the following reaction sequence: (see article) The rate of appearance of fluorescence of NADH (lambdaex = 365 nm, lambdaem = 460 nm), developed in the indicator reaction (4), is measured and equated to the activity of alpha-amylase in serum. A calibration plot of the change of fluorescence per min vs. enzyme concentration shows a good proportionality in the range of 0.50-5.0 kU/liter.


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Copyright © 1976 by the American Association for Clinical Chemistry.