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Clinical Chemistry 22: 1727-1728, 1976;
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Clinical Chemistry, Vol 22, 1727-1728, Copyright © 1976 by American Association for Clinical Chemistry

Bile acid and detergent interaction with radioassays based on coated charcoal

KJ Andersen, H Schjonsby, KW Skagen and G von der Lippe

We tested the radioassays for cyclic AMP and human gastrin, both involving separation on coated charcoal, for interaction with bile acids and detergents, and found a concentration-dependent interaction of taurocholic and glycocholic acid as well as of the surfactant Triton X-100 and sodium laurylsulfate in both assays. The interaction was detectable from concentrations of 0.5 mmol of bile acid per liter or 625 mg of detergent per liter, giving rise to falsely decreased gastrin values and falsely increased or decreased values for cyclic AMP. The interactions demonstrated may be a general effect on all radioassays that are based on the use of coated charcoal in the separation of free from bound ligands.




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Copyright © 1976 by the American Association for Clinical Chemistry.