Clinical Chemistry
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Clinical Chemistry 22: 1729-1731, 1976;
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Clinical Chemistry, Vol 22, 1729-1731, Copyright © 1976 by American Association for Clinical Chemistry

Interference by acetaminophen in the glucose oxidase-peroxidase method for blood glucose determination

I Kaufmann-Raab, HG Jonen, E Jahnchen, GF Kahl and U Groth

Acetaminophen, p-aminophenol, and oxyphenbutazone interfere with the glucose oxidase/peroxidase method for glucose. Structurally related compounds that lack a free phenolic hydroxyl group (acetanilide, aniline, and phenylbutazone) do not interfere. During the analytical procedure acetaminophen is consumed. One mole of acetaminophen leads to an apparent loss of four moles of glucose. The hexokinase/glucose-6- phosphate dehydrogenase method (Boehringer Hexokinase method) is not affected by these substances.


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Copyright © 1976 by the American Association for Clinical Chemistry.