Clinical Chemistry
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Clinical Chemistry 22: 1909-1912, 1976;
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Clinical Chemistry, Vol 22, 1909-1912, Copyright © 1976 by American Association for Clinical Chemistry

Error introduced by specimen handling before determination of inorganic phosphate concentrations in plasma and serum

JE Carothers, NM Kurtz and J Lemann Jr

Values for serum inorganic phosphate (Pi) concentrations in groups of healthy adults vary widely, the coefficient of variation ranging from 10 to 15%. We undertook to determine in 23 healthy adults whether part of this variation could be accounted for by (a) drawing blood in syringes vs. evacuated tubes (b) the time between blood sampling and separation of serum or plasma, and (c) the prevention of clotting. Values were unaffected by a, decreased significantly with time at room temperature between blood sampling and separation of cells in both serum and plasma, and were significantly lower in plasma than in serum. The group coefficient of variation for Pi averaged 13% and was uninfluenced by the blood-processing technique.




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Copyright © 1976 by the American Association for Clinical Chemistry.