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Clinical Chemistry 22: 1973-1975, 1976;
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Clinical Chemistry, Vol 22, 1973-1975, Copyright © 1976 by American Association for Clinical Chemistry

Serum phospholipids determined without acid digestion

RS Sandhu

A method for determination of phospholipids in serum is described that involves complexing intact phospholipids with chromogenic reagent, thus eliminating the need for acid digestion, which is a mandatory step in all the current procedures for phospholipid analysis. Inorganic phosphorus and other serum components do not interfere in the determination. Excellent agreement between results of this new procedure and the conventional acid digestion method (r=0.986, y intercept=2.053, and slope=0.986) makes it an attractive alternative clinical method for determination of phospholipids in serum.




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Copyright © 1976 by the American Association for Clinical Chemistry.