Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 22: 2006-2009, 1976;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gitlitz, P.
Right arrow Articles by Frings, C.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Gitlitz, P.
Right arrow Articles by Frings, C.

Clinical Chemistry, Vol 22, 2006-2009, Copyright © 1976 by American Association for Clinical Chemistry

Interferences with the starch-iodine assay for serum amylase activity, and effects of hyperlipemia

PH Gitlitz and CS Frings

Recent reports imply or claim that amylase activity is inhibited in hyperlipemic sera, because diluted samples showed greater activities when assayed by a starch-iodine method. We find that dilutions of both clear and lipemic samples give higher-than-expected activities when assayed by a starch-iodine method, an effect attributable to the variable effects of protein, turbidity, and triglycerides in the hyperlipemic samples. Thus the starch-iodine method is unsuitable for assessing the effects of hyperlipemic samples on amylase activity. To do so, we used an alternative method, in which soluble dyed amylopectin is used as the substrate. This method exhibits apparent zero-order kinetics, and we detected no interfering factors. Plots of sample volume (x) vs. activity (y) for clear and hyperlipemic (triglycerides up to 80 g/liter) sera gave straight lines with y-intercepts of zero. Evidently hypertriglyceridemia does not inhibit amylase activity.


The following articles in journals at HighWire Press have cited this article:


Home page
 J ANIM SCIHome page
N. Liu, Y. J. Ru, F. D. Li, and A. J. Cowieson
Effect of diet containing phytate and phytase on the activity and messenger ribonucleic acid expression of carbohydrase and transporter in chickens
J Anim Sci, December 1, 2008; 86(12): 3432 - 3439.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1976 by the American Association for Clinical Chemistry.