Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 22: 2025-2028, 1976;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bais, R
Right arrow Articles by Edwards, J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Bais, R
Right arrow Articles by Edwards, J.

Clinical Chemistry, Vol 22, 2025-2028, Copyright © 1976 by American Association for Clinical Chemistry

An optimized continuous-monitoring procedure for semiautomated determination of serum acid phosphatase activity

R Bais and JB Edwards

A continuous-monitoring method for measuring acid phosphatase activity with alpha-naphthyl phosphate as the substrate was critically evaluated and modified. Using partially purified prostatic acid phosphatase, we show that certain conditions for the assay must be satisfied to ensure linearity. These conditions include maintaining the pH between 5.6 and 5.9 and the addition of detergent to sustain linearity. The results obtained with alpha-naphthyl phosphate have been compared with those obtained by using p-nitrophenyl phosphate as substrate. When used with an automatic rate analyzer, the modified method is as sensitive but more reproducible.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
M. Nakanishi, K. Yoh, K. Uchida, S. Maruo, and A. Matsuoka
Improved method for measuring tartrate-resistant acid phosphatase activity in serum
Clin. Chem., February 1, 1998; 44(2): 221 - 225.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1976 by the American Association for Clinical Chemistry.