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Clinical Chemistry 22: 2025-2028, 1976;
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Clinical Chemistry, Vol 22, 2025-2028, Copyright © 1976 by American Association for Clinical Chemistry

An optimized continuous-monitoring procedure for semiautomated determination of serum acid phosphatase activity

R Bais and JB Edwards

A continuous-monitoring method for measuring acid phosphatase activity with alpha-naphthyl phosphate as the substrate was critically evaluated and modified. Using partially purified prostatic acid phosphatase, we show that certain conditions for the assay must be satisfied to ensure linearity. These conditions include maintaining the pH between 5.6 and 5.9 and the addition of detergent to sustain linearity. The results obtained with alpha-naphthyl phosphate have been compared with those obtained by using p-nitrophenyl phosphate as substrate. When used with an automatic rate analyzer, the modified method is as sensitive but more reproducible.


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 Clin. Chem.Home page
M. Nakanishi, K. Yoh, K. Uchida, S. Maruo, and A. Matsuoka
Improved method for measuring tartrate-resistant acid phosphatase activity in serum
Clin. Chem., February 1, 1998; 44(2): 221 - 225.
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