Clinical Chemistry
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Clinical Chemistry 22: 2042b-2045b, 1976;
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Clinical Chemistry, Vol 22, 2042-2045, Copyright © 1976 by American Association for Clinical Chemistry

Automated enzymatic determination of L-lactate in serum, with use of a miniature centrifugal analyzer

TP Hadjiioannou, SI Hadjiioannou, SD Brunk and HV Malmstadt

We describe an automated enzymatic reaction-rate method for spectrophotometric determination of lactate in serum with a miniature centrifugal analyzer. The L(+) -lactate is selectively oxidized in the presence of lactate dehydrogenase (EC 1.1.1.27) and NAD+ to form NADH, which is measured from its absorption. Reaction rates are determined automatically, and unknown concentrations are calculated from a computer-generated calibration curve with aqueous lithium lactate standards. Lactate concentrations in the range 0.32-1.6 mug/4 mul (80- 400 mg/liter) of sample were determined with relative errors and coefficient of variation of 4.8%. Analytical recovery of lactate added to pooled serum was 89-112% (average, 101%). Comparison with a kit ("Rapid Lactate") method gave a correlation coefficient squared of 0.979 over a concentration range of 39-779 mg/liter.




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Copyright © 1976 by the American Association for Clinical Chemistry.