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Clinical Chemistry 22: 169-172, 1976;
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Clinical Chemistry, Vol 22, 169-172, Copyright © 1976 by American Association for Clinical Chemistry

Colorimetry of carbohydrates with m-aminophenol, and its proposed use in measurement of serum amylase activity

JF Goodwin

In the reaction of m-aminophenol with carbohydrates in a thiourea/acetic acid reagent, addition of borate suppresses chromogen formation for hexoses and pentoses, and enhances it for disaccharides. All reaction products absorb maximally at 520 nm. The relatively greater intensity of the m-aminophenol/maltose reaction product was used in a saccharogenic method for amylase, with low blank readings. This method is compared with two dye/starch procedures.




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Copyright © 1976 by the American Association for Clinical Chemistry.