Clinical Chemistry
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Clinical Chemistry 22: 224-226, 1976;
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Clinical Chemistry, Vol 22, 224-226, Copyright © 1976 by American Association for Clinical Chemistry

Cholesterol in amniotic fluid, determined by gas chromatography

VM Jones Owen, FK Ho, A Mazzuchin, TA Doran, S Liedgren and CJ Porter

Cholesterol was extracted from amniotic fluid, saponified, converted to its trimethylsilyl derivative, and gas chromatographed, with cholesteryl acetate as the internal standard. The method is sufficiently accurate and precise for use with the range of concentrations of cholesterol found in amniotic fluid (5 to 48 mg/litre). Total cholesterol was measured in amniotic fluids collected at different stages of gestation. No significant trend or change was observed nor was cholesterol in the amniotic fluid and the mother's serum correlated at any stage of gestation. Thus we conclude that cholesterol is not a useful indicator of fetal age or maturity. Cholesterol concentrations in amniotic fluid from complicated pregnancies were within the range found for normal pregnancies.




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Copyright © 1976 by the American Association for Clinical Chemistry.