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Clinical Chemistry 22: 232-234, 1976;
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Clinical Chemistry, Vol 22, 232-234, Copyright © 1976 by American Association for Clinical Chemistry

Improved assay for urinary 17-hydroxycorticosteroids

Y Ariyoshi and Y Osawa

We describe a simple, rapid, reliable method for determining urinary 17- hydroxycorticosteroids. A neutral resin (Amberlite XAD-2), which is a non-ionic cross-linked polystyrene, is used to extract and concentrate the steroids, which are then quantitatively determined with the Porter- Silber reaction. Use of the resin eliminates the need for enzymatic hydrolysis and n-butanol extractions, thereby decreasing analysis time considerably; results can be obtained within 3 h of receipt of specimens. Most of the nonsteroidal Porter-Silber chromogens are removed, resulting in a method that is highly specific and sensitive, even at low concentrations (0.6 mg/liter), and so more accurate and reproducible than currently used methods.




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Copyright © 1976 by the American Association for Clinical Chemistry.