Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 22: 243-245, 1976;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Forrester, R.
Right arrow Articles by Pierre, K.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Forrester, R.
Right arrow Articles by Pierre, K.

Clinical Chemistry, Vol 22, 243-245, Copyright © 1976 by American Association for Clinical Chemistry

Enzymatic method for determination of CO2 in serum

RL Forrester, LJ Wataji, DA Silverman and KJ Pierre

We describe an enzymatic method, requiring only 10 mul of serum, for determining CO2 as bicarbonate or dissolved gas. Phosphoenolpyruvate carboxylase catalyzes the reaction of HCO3- with phosphoenolypyruvate to give oxalacetate. The resulting NADH, in the presence of malate dehydrogenase, is oxidized to NAD+, and the decrease in absorbance at 340 nm is directly proportional to the amount of CO2 present in the sample. Reaction is complete in 3 to 6 min under assay conditions, and is linearly related to CO2 concentrations between 8 and 65 mmol/liter. Analytical recovery is 95-110% (average, 101%). Two laboratories compared values obtained by continuous-flow analysis. The resulting correlation coefficients were 0.966 and 0.987, values for the t-test were t(paired) equals 0.473 and t(paired) equals 0.334, and average day- to-day precisions (three concentrations) were 3.9% and 4.2%.


The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
I. Lopez-Flores, J. Peragon, R. Valderrama, F. J. Esteban, F. Luque, M. A. Peinado, F. Aranda, J. A. Lupianez, and J. B. Barroso
Downregulation in the expression of the serine dehydratase in the rat liver during chronic metabolic acidosis
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2006; 291(5): R1295 - R1302.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Zhou, J. Zhao, P. Bouyer, and W. F. Boron
Evidence from renal proximal tubules that HCO3 and solute reabsorption are acutely regulated not by pH but by basolateral HCO3 and CO2
PNAS, March 8, 2005; 102(10): 3875 - 3880.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Zhao, Y. Zhou, and W. F. Boron
Effect of isolated removal of either basolateral HCO-3 or basolateral CO2 on HCO-3 reabsorption by rabbit S2 proximal tubule
Am J Physiol Renal Physiol, August 1, 2003; 285(2): F359 - F369.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1976 by the American Association for Clinical Chemistry.