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Clinical Chemistry 22: 257-260, 1976;
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Clinical Chemistry, Vol 22, 257-260, Copyright © 1976 by American Association for Clinical Chemistry

Improved method for measurement of inorganic phosphate in serum with a centrifugal analyzer

PW Wentz, J Savory and RE Cross

A direct mehtod [Clin. Chim. Acta 46, 113 (1973)] for determination of inorganic phosphate in serum was adapted for use with a centrifugal analyzer. Contamination is minimized and analysis rate maximized by doing the reaction in the reagent wells of the transfer disc and by utilizing the high-speed spectrophotometric and data-reduction capabilities of the centrifugal analyzer. Hemolysis, icterus, and moderate lipemia cause no interference. Grossly lipemic sera and sera from patients with plasma cell dyscrasias can be analyzed by incorporating appropriate blanking and dilution techniques. The method exhibits excellent sensitivity and precision and results correlate well with those from a continuous-flow procedure.




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Copyright © 1976 by the American Association for Clinical Chemistry.