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Clinical Chemistry 22: 261-263, 1976;
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Clinical Chemistry, Vol 22, 261-263, Copyright © 1976 by American Association for Clinical Chemistry

Parallel electrophoretic fractionation of alkaline phosphatase and serum protein on cellulose acetate strips: clinical evaluation

A Burlina and L Galzigna

We describe here a simple, time-saving method for separating alkaline phosphatases on cellulose acetate, with a parallel detection of serum protein pattern. The staining procedure is based on a coupling reaction between the reaction product of alpha-naphthol with Fast Red Violet LB. The enzymatic reaction is done by bringing the cellulose acetate strip containing the phosphatase band into contact with the surface of a solid agar/substrate mixture. The whole procedure requires 80 min. The method has some features in common with other recently proposed procedures but is more reproducible and rapid because of the choice of the reagents and an improved yield of the coupling step.




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Copyright © 1976 by the American Association for Clinical Chemistry.