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Clinical Chemistry 22: 445-448, 1976;
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Clinical Chemistry, Vol 22, 445-448, Copyright © 1976 by American Association for Clinical Chemistry

Stability of fatty acids in hyperlipoproteinemic plasma during long- term storage

EZ Hirsch, S Slivka and AP Gibbons

Aliquots of hyperlipoproteinemic plasma were stored at -20 degrees C in a nitrogen atmosphere with added disodium ethylenediaminetetraacetate for up to one year, to determine the stability of fatty acids in the four major lipid classes under these conditions. Assays were performed at zero time and at 2, 5, and 12 months. No uniform fatty acid change was found. Minor statistically significant changes were found in total triglyceride fatty acids and in some of the individual fatty acids in cholesterol esters, phospholipid, and free fatty acids. These minor changes could not be accounted for by plasma lipid concentrations, lipolysis, or fatty acid peroxidation. Evidently, plasma can be stored in this manner for as long as a year without substantial change in fatty acids in any of the four major lipid classes.




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Copyright © 1976 by the American Association for Clinical Chemistry.