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Clinical Chemistry 22: 468-470, 1976;
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Clinical Chemistry, Vol 22, 468-470, Copyright © 1976 by American Association for Clinical Chemistry

Automated separation and measurement of lactate dehydrogenase isoenzymes

MG Busby, PJ Kudirka, RN Carey and EC Toren Jr

We describe a totally automated, computer-controlled system for separating and measuring the activity of lactate dehydrogenase (EC 1.1.1.27) isoenzymes. These isoenzymes in tissue extracts were separated on disposable DEAE-cellulose mini-columns. Resolution was complete except that LD-1 was not resolved from LD-2.




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Copyright © 1976 by the American Association for Clinical Chemistry.