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Clinical Chemistry 22: 611-615, 1976;
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Clinical Chemistry, Vol 22, 611-615, Copyright © 1976 by American Association for Clinical Chemistry

Rapid radioimmunoassay of total urinary estriol

DW Anderson and U Goebelsmann

We describe a radioimmunoassay for total urinary estriol in pregnancy. A 25-mul aliquot of the urine specimen is acid hydrolyzed, neutralized, and diluted before assay. We use rabbit antisera against estriol-6-(O- carboxymethyl)oxime/bovine serum albumin and ammonium sulfate precipitation at room temperature. Results are unaffected by glucose or methenamine mandelate, a urinary tract antiseptic. Using semi-automatic pipetting equipment, one laboratory technologist can complete 50 assays within 8 h. This technique is both reliable and convenient and should decrease the expense of routine estriol assays.


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Copyright © 1976 by the American Association for Clinical Chemistry.