Clinical Chemistry
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Clinical Chemistry 22: 681-682, 1976;
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Clinical Chemistry, Vol 22, 681-682, Copyright © 1976 by American Association for Clinical Chemistry

Specificity and reproducibility of acetone precipitation in identifying surface-active phosphatidylcholine in amniotic fluid

LL Penney, DD Hagerman and CA Sei

We show the thoretical and actual variability in the amount of surface- active lecithin precipitated in the acetone-precipitation step of determining lecithin/sphingomyelin (L/S) ratios in the clinical laboratory. Lecithins precipitated may range from 60 to 90% of the total surface-active lecithins present. The range is sufficient to account for the occasional "immature" L/S ratios (less than 2/1) found in amniotic fluid from women bearing mature fetuses.




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Copyright © 1976 by the American Association for Clinical Chemistry.