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Clinical Chemistry 22: 785-788, 1976;
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Clinical Chemistry, Vol 22, 785-788, Copyright © 1976 by American Association for Clinical Chemistry

Enzymatic assay for methotrexate in serum and cerebrospinal fluid

LC Falk, DR Clark, SM Kalman and TF Long

We describe an assay for methotrexate in biological fluids. The assay is based on inhibition by methotrexate of dihydrofolate reductase from Lactobacillus casei. The lower limit of sensitivity in serum is 2 X 10(- 8) mol (about 10 mug) of methotrexate per liter. Within-run precision is +/- 5% (coefficient of variation) and day-to-day variation is 18%. Advantages of the assay are ease of manipulation, high sensitivity, and specificity.


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Copyright © 1976 by the American Association for Clinical Chemistry.