Clinical Chemistry
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Clinical Chemistry 22: 806-809, 1976;
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Clinical Chemistry, Vol 22, 806-809, Copyright © 1976 by American Association for Clinical Chemistry

Gas-chromatographic determination of an antifibrinolytic drug, epsilon- aminocaproic acid

TR Keucher, EB Solow, J Metaxas and RL Campbell

We describe a modified method for assay of epsilon-aminocaproic acid. Serum or cerebrospinal fluid is deproteinized, followed by cation- exchange column-chromatography, and N-trifluoroacetyl-n-butyl derivatives of amino acids are formed and separated by gas chromatography. Tranexamic acid, a nonprotein amino acid, was used as an internal standard. The assay is sensitive and precise, and results correlate adequately with those obtained with an automated amino acid analyzer (ion-exchange chromatography).




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Copyright © 1976 by the American Association for Clinical Chemistry.