Clinical Chemistry
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Clinical Chemistry 22: 817-823, 1976;
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Clinical Chemistry, Vol 22, 817-823, Copyright © 1976 by American Association for Clinical Chemistry

Determination of clomipramine and desmethyl-clomipramine in plasma or urine by the double-radioisotope derivative technique

G Carnis, J Godbillon and JP Metayer

A double radioisotope derivative method was developed for the determination of clomipramine and desmethyl-clomipramine in plasma or urine. After addition of 14C-labeled clomipramine and desmethyl- clomipramine as internal standards and extractive isolation of both compounds, desmethyl-clomipramine is acetylated with [3H]acetic anhydride. The [3H]acetamide is separated from clomipramine by thin- layer chromatography and its radioactivity is measured. Clomipramine, extracted from the cilica gel, is reacted with trichloroethyl chloroformate. The urethane is saponified and decarboxylated. The resulting desmethyl-clomipramine is acetylated with [3H]acetic anhydride. The [3H]acetamide is purified by thin-layer chromatography and its radioactivity is measured. The sensitivity of the method is 15 mug/liter for clomipramine and 2 mug/liter for desmethyl-clomipramine. Its specificity was made sure by a cross-check with a gas chromatography-mass spectrometry technique.


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A.H. Stead and A.C. Moffat
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Human and Experimental Toxicology, January 1, 1983; 2(3): 437 - 464.
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Copyright © 1976 by the American Association for Clinical Chemistry.