Clinical Chemistry
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Clinical Chemistry 22: 1066-1069, 1976;
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Clinical Chemistry, Vol 22, 1066-1069, Copyright © 1976 by American Association for Clinical Chemistry

Simple determination of 3alpha-hydroxy fraction and 3beta-hydroxy fraction of urinary 17-ketosteroids

Y Yamaguchi, C Hayashi and Y Kumahara

We describe a method for determination of the 3alpha-hydroxysteroid fraction and 3beta-hydroxysteroid fraction of 17-ketosteroids in urine. The method includes enzymatic hydrolysis with beta-glucuronidase and extraction with ethyl acetate and color development of 3beta- hydroxysteroids by 3beta-hydroxysteroid oxidase and of total 17- ketosteroids by the Zimmermann reaction. The value for 3alpha- hydroxysteroids is calculated by subtracting the value for 3beta- hydroxysteroids from the value for total 17-ketosteroids. Recovery was assessed and results compared with those by an accepted method. Results were satisfactory for measuring these fractions of 17-ketosteroids.




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Copyright © 1976 by the American Association for Clinical Chemistry.