Clinical Chemistry
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Clinical Chemistry 22: 1098-1099, 1976;
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Clinical Chemistry, Vol 22, 1098-1099, Copyright © 1976 by American Association for Clinical Chemistry

Effect of reaction initiator on human lactate dehydrogenase assay

SN Buhl, KY Jackson, R Lubinski and RE Vanderlinde

Human lactate dehydrogenase isoenzymes I and V have decreased activities when the reaction is initiated with lactate. No loss in lactate dehydrogenase I activity was found when the reaction was initiated with enzyme or NAD+. For lactate dehydrogenase V an NAD+- initiated reaction, as compared to an enzyme-initiated reaction, yields lower activity in sodium pyrophosphate buffer but higher activity in tris(hydroxymethyl)aminomethane buffer. Both isoenzymes have higher lactate-to-pyruvate activity when assayed in the latter buffer than when assayed in the former. Human lactate dehydrogenase V (but not I) exhibited different activities when assayed with lactate from two different commercial sources. Human lactate dehydrogenase assayed by the pyruvate-to-lactate reaction is not affected by the choice of reaction initiator.




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Copyright © 1976 by the American Association for Clinical Chemistry.