Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 22: 972-976, 1976;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Belle, H
Right arrow Search for Related Content
PubMed
Right arrow Articles by Van Belle, H

Clinical Chemistry, Vol 22, 972-976, Copyright © 1976 by American Association for Clinical Chemistry

Alkaline phosphatase. I. Kinetics and inhibition by levamisole of purified isoenzymes from humans

H Van Belle

I studied the kinetics and sensitivity toward inhibition by levamisole and R 8231 of the most important human alkaline phosphatase isoenzymes. N-Ethylaminoethanol proved superior to the now widely used diethanolamine buffer, especially for the enzymes from the intestine and placenta, behaving as an uncompetitive activator. The optimum pH largely depends on the substrate concentration. The addition of Mg2+ has no effect on the activities. The meaning of Km-values for alkaline phosphatases is questioned. Isoenzymes from human liver, bone, kidney, and spleen are strongly inhibited by levamisole or R 8231 at concentrations that barely affect the enzymes from intestine or placenta. The inhibition is stereospecific, uncompetitive, and not changed by Mg2+. Inhibition is counteracted by increasing concentrations of N-ethylaminoethanol. The mechanism of inhibition is suggested to be formation of a complex with the phosphoenzyme.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Biol. Chem.Home page
P. Zuo, M. Picher, S. F. Okada, E. R. Lazarowski, B. Button, R. C. Boucher, and T. C. Elston
Mathematical Model of Nucleotide Regulation on Airway Epithelia: IMPLICATIONS FOR AIRWAY HOMEOSTASIS
J. Biol. Chem., September 26, 2008; 283(39): 26805 - 26819.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
K. Chen, K. Wang, A. M. Kirichian, A. F. Al Aowad, L. K. Iyer, S. J. Adelstein, and A. I. Kassis
In silico design, synthesis, and biological evaluation of radioiodinated quinazolinone derivatives for alkaline phosphatase-mediated cancer diagnosis and therapy
Mol. Cancer Ther., December 1, 2006; 5(12): 3001 - 3013.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
P. Koszalka, B. Ozuyaman, Y. Huo, A. Zernecke, U. Flogel, N. Braun, A. Buchheiser, U. K.M. Decking, M. L. Smith, J. Sevigny, et al.
Targeted Disruption of cd73/Ecto-5'-Nucleotidase Alters Thromboregulation and Augments Vascular Inflammatory Response
Circ. Res., October 15, 2004; 95(8): 814 - 821.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Picher, L. H. Burch, A. J. Hirsh, J. Spychala, and R. C. Boucher
Ecto 5'-Nucleotidase and Nonspecific Alkaline Phosphatase. TWO AMP-HYDROLYZING ECTOENZYMES WITH DISTINCT ROLES IN HUMAN AIRWAYS
J. Biol. Chem., April 4, 2003; 278(15): 13468 - 13479.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1976 by the American Association for Clinical Chemistry.