Clinical Chemistry
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Clinical Chemistry 22: 1310-1313, 1976;
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Clinical Chemistry, Vol 22, 1310-1313, Copyright © 1976 by American Association for Clinical Chemistry

Simplified, totally enzymatic method for determination of serum triglycerides with a centrifugal analyzer

SH Grossman, E Mollo and G Ertingshausen

We describe a totally enzymatic method for determination of serum triglycerides (triacylglycerols) specifically adaptable to the CentrifiChem system. The method involves lipolysis with lipase from Rhizopus arrhizus alone and quantitation of the resulting glycerol with glycerol dehydrogenase in a kinetic, fixed-time mode. Hydrolysis by the lipase is complete, for concentrations up to at least 5.0 g/liter, in 10 min at room temperature. The unfavorable equilibrium for the oxidation of glycerol is overcome by increasing the pH and adding excess NAD+. Under these conditions the glycerol determination is linear to at least 4.0 g of glycerol per liter, as triglyceride. The test exhibits acceptable accuracy and precision, and results correlate well with those by an alternative totally enzymatic procedure. The present method is unaffected by phosphatase and a considerably simplified reagent is used.


The following articles in journals at HighWire Press have cited this article:


Home page
 JPEN J Parenter Enteral NutrHome page
M. M. Meguid, M. Kurzer, R. J. Hayashi, and M. P. Akahoshi
Short-Term Effects of Fat Emulsion on Serum Lipids in Postoperative Patients
JPEN J Parenter Enteral Nutr, January 1, 1989; 13(1): 77 - 80.
[Abstract] [PDF]


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Copyright © 1976 by the American Association for Clinical Chemistry.