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Clinical Chemistry 22: 1351-1354, 1976;
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Clinical Chemistry, Vol 22, 1351-1354, Copyright © 1976 by American Association for Clinical Chemistry

Measuring water content of feces by the Karl Fischer method

R Jensen, D Buffangeix and G Covi

We describe a technique for measuring the water content of stools by the Karl Fischer method. The analysis is based on removal of water into a mixture of methanol/chloroform (1/2), after dispersion of the stool by sonication in presence of solvent. An aliquot of the solution thus obtained is placed in themeasuring cell of a Karl Fischer apparatus and then analyzed in the classic way. We further describe the advantages of this method (odorless, precise, reproducible) in contrast to other current methods. In addition the same organic solution can also be used in determining the lipid content of stools.


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 Clin. Chem.Home page
A. Rivero-Marcotegui, J. E. Olivera-Olmedo, F. S. Valverde-Visus, M. Palacios-Sarrasqueta, A. Grijalba-Uche, and S. Garcia-Merlo
Water, fat, nitrogen, and sugar content in feces: reference intervals in children
Clin. Chem., July 1, 1998; 44(7): 1540 - 1544.
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Copyright © 1976 by the American Association for Clinical Chemistry.