Clinical Chemistry
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Clinical Chemistry 22: 1394-1396, 1976;
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Clinical Chemistry, Vol 22, 1394-1396, Copyright © 1976 by American Association for Clinical Chemistry

Improved continuous-flow method for detemination of total serum hexosamines

PZ Sobocinski, WJ Canterbury and KH Jurgens

We describe an automated determination of serum hexosamine by the Elson- Morgan reaction, together with reagent modifications that minimiz interference from amino acids and sugars present in acid hydrolysates of sera. We used a novel 15-min autoclave procedure for the acid hydrolysis of sera before analysis to facilitate the detemination as compared to the 4-h hydrolysis used in the conventional manual method. Results correlated well (r = 0.906) with those obtained by the corresponding manual method.




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Copyright © 1976 by the American Association for Clinical Chemistry.