Clinical Chemistry
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Clinical Chemistry 23: 2142-2144, 1977;
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Clinical Chemistry, Vol 23, 2142-2144, Copyright © 1977 by American Association for Clinical Chemistry

A solid-phase enzyme immunoassay for serum ferritin

L Theriault and M Page

We describe an enzyme immunoassay for human serum ferritin in which antibody adsorbed on polystyrene tubes is used. Adsorbed gamma- globulins against human ferritin were first allowed to react with ferritin and a second antiferritin antibody, labeled with alkaline phosphatase, was added. The amount of bound enzyme/antibody conjugate was proportional to the ferritin titer in the assay. This method offers stable reagents that can be kept for many months at 4 degrees C. The average values for ferritin in normal men and women were, respectively, 58 and 43 microgram/liter. The lowest detectable concentration was 5 microgram/liter.




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Copyright © 1977 by the American Association for Clinical Chemistry.