Clinical Chemistry
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Clinical Chemistry 23: 2311-2323, 1977;
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Clinical Chemistry, Vol 23, 2311-2323, Copyright © 1977 by American Association for Clinical Chemistry

Inhibition by concanavalin A as the basis for a specific assay of serum 5'-nucleotidase activity

ER Zygowicz, FW Sunderman Jr, E Horak and JF Dooley

Concanavalin A inhibits serum 5'-nucleotidase activity, without causing significant inhibition of alkaline phosphatase activity. This observation serves as the basis for a new method for assaying the 5'- nucleotidase activity in serum, which depends upon the difference between the enzymic hydrolysis of adenosine-5'-monophosphate in the presence and absence of concanavalin A. A denosine released by the 5'- nucleotidase reaction is deaminated by a coupled reaction with adenosine deaminase to liberate inosine and ammonia, and ammonia is measured colorimetrically by the Berthelot reaction. In sera from 40 healthy adult persons, 5'-nucleotidase activity averaged 6.4 U/liter (SD, +/-2.0; range, 3-12). In sera from 100 patients, measurements of 5'-nucleotidase activity by the new assay averaged 8% lower than by a generally accepted method in which phenyl phosphate is used to suppress hydrolysis of adenosine-5'-monophosphate by alkaline phosphatase activity. The clinical validy of the new assay was tested by measuring serum 5'-nucleotidase activities in rats with bile duct ligation and in rats treated with thioacetamide to induce hepatocellular injury.


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M. Kukley, P. Stausberg, G. Adelmann, I. P. Chessell, and D. Dietrich
Ecto-Nucleotidases and Nucleoside Transporters Mediate Activation of Adenosine Receptors on Hippocampal Mossy Fibers by P2X7 Receptor Agonist 2'-3'-O-(4-Benzoylbenzoyl)-ATP
J. Neurosci., August 11, 2004; 24(32): 7128 - 7139.
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Copyright © 1977 by the American Association for Clinical Chemistry.