Clinical Chemistry
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Clinical Chemistry 23: 2324-2328, 1977;
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Clinical Chemistry, Vol 23, 2324-2328, Copyright © 1977 by American Association for Clinical Chemistry

Radioimmunoassay for normalized thyroxine with use of Sephadex columns

WT Goedemans, PC Bartels and AF Roijers

We describe a reproducible radioimmunoassay, with use of Sephadex columns, for measuring normalized thyroxine. In comparison with a competitive protein-binding procedure, the present method is more specific because antibody rather than thyroxine-binding globulin is responsible for the competition between endogeneous and tracer thyroxine. In barbital buffer, various concentrations of thyroxine binding pre-albumin and albumin had no influence on the results. Values obtained by the present method correlated well with those by the free thyroxine index and a generally accepted thyroxine normalized method.




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Copyright © 1977 by the American Association for Clinical Chemistry.