Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 23: 280-282, 1977;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pesce, M. A.
Right arrow Articles by Bodourian, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pesce, M. A.
Right arrow Articles by Bodourian, S. H.

Clinical Chemistry, Vol 23, 280-282, Copyright © 1977 by American Association for Clinical Chemistry

Enzymic measurement of cholesterol in serum with the CentrifiChem centrifugal analyzer

MA Pesce and SH Bodourian

Cholesterol is measured by mixing 5 mul of sample with 350 mul of a reagent consisting of phenol, 4-aminoantipyrine, and the enzymes cholesterol oxidase, cholesterol esterase, and peroxidase. After 12 min, the resulting quinoneimine is measured at 520 nm. Readings and cholesterol concentrations are linearly related up to 4.0 g/liter. Lipemic sera and samples containing uric acid (up to 200 mg/liter), hemoglobin (up to 1 g/liter), and certain drugs (clofibrate, phenobarbital, nicotinic acid, Ketochol, Ovral-28), gave no interference. Abnormally high concentrations of bilirubin and ascorbic acid in serum lowered the cholesterol values. This enzymic assay, compared with the method of Abell and with a rate method that uses the Hantzsch reaction, gave correlation coefficients of 0.987 and 989, respectively.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1977 by the American Association for Clinical Chemistry.