Clinical Chemistry
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Clinical Chemistry 23: 464-468, 1977;
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Clinical Chemistry, Vol 23, 464-468, Copyright © 1977 by American Association for Clinical Chemistry

Extraction and measurement of circulating angiotensins I and II

JD Barrett, P Eggena and MP Sambhi

We describe a method for simultaneous extraction of angiotensins I and II from human blood. Blood was diluted with 10 volumes of isotonic saline containing ammonium ethylenediaminetetraacetate, and the angiotensin peptides were extracted by cation-exchange chromatography. The dilution effectively inhibits in vitro production of angiotensin I, conversion of angiotensin I to angiotensin II, and degradation of the peptides. Analytical recovery of exogenous angiotensin I or II added to whole blood in physiological concentrations was essentially complete. The extraction procedure removes blood and plasma components that interfere with radioimmunoassay of angiotensin, thus eliminating the need to correct for nonspecific interference. Circulating angiotensins I and II, measured in patients with essential hypertension before and after a week of diuretic administration, increased in parallel with plasma renin activity.


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 Am. J. Physiol. Heart Circ. Physiol.Home page
S. G. Lage, L. Kopel, C. C. J. Medeiros, R. T. Carvalho, and M. A. Creager
Angiotensin II contributes to arterial compliance in congestive heart failure
Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1424 - H1429.
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Copyright © 1977 by the American Association for Clinical Chemistry.