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Clinical Chemistry 23: 571-575, 1977;
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Clinical Chemistry, Vol 23, 571-575, Copyright © 1977 by American Association for Clinical Chemistry

Nonenzymic spectrophotometric determination of potential poison ivy cross-reactors

AJ Quattrone

I describe an inexpensive, nonenzymic analytical system for prescreening substances that might cross-react as Rhus toxing (e.g., poison ivy, poison oak, and sumac allergens) on human skin. By spectrophotometric assay after incubation with an oxidizing mixture of Cu(II)ammine complex and ammonium persulfate, I could accurately and reproducibly determine o-quinoidal products of several potential synthetic cross-reactors and native poison ivy allergen, and could distinguish these from catecholamines, resorcinol, p-hydroquinone, and a closely related phenol. A good correlation was obtained between this nonenzymic technique and an enzymic assay. This Cu(II)ammine/persulfate oxidative assay, however, is inexpensive and obviates any spectral interference from enzymic proteins.




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Copyright © 1977 by the American Association for Clinical Chemistry.