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Clinical Chemistry 23: 796-801, 1977;
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Clinical Chemistry, Vol 23, 796-801, Copyright © 1977 by American Association for Clinical Chemistry

Evaluation of the radioimmunoassay for benzoylecgonine (a cocaine metabolite) in human urine

SJ Mule, D Jukofsky, M Kogan, A De Pace and K Verebey

The 125I-radioimmunoassay (RIA) for benzylecgonine (cocaine metabolite) in urine was evaluated by comparison with gas-liquid chromatography (GLC) and thin-layer chromatography (TLC) and the Enzyme-Multiplied Immunoassay Technique (EMIT). By RIA, a statistically significant concentration, 2 microng/liter, was observed for urinary benzoylecgonine. The coefficient of variation for the RIA was 2.58+/- 0.38% inter-assay and 2.20+/-0.14% intraassay. There was cross- reactivity with cocaine (more reactive than benzoylecgonine) and other members of the tropane family of alkaloids. There was agreement between results by RIA and GLC in 95.5% of the samples, between RIA and TLC in 87.0%, and between RIA and EMIT in 84.5%. The percentage of true false- positives was 3.5% for the RIA in comparison to GLC, 8.8% in comparison to TLC, and 9.1% in comparison to EMIT. True false-negatives were insignificant (0 to 1.0%). GLC and RIA results correlated highly (phi=0.908). GLC, therefore, was the best comparison method for this evaluation study. RIA for benzoylecgonine is sensitive, reproducible, and reliable for the detection of cocaine in urine.


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[Abstract]


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Copyright © 1977 by the American Association for Clinical Chemistry.