Clinical Chemistry
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Clinical Chemistry 23: 802-805, 1977;
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Clinical Chemistry, Vol 23, 802-805, Copyright © 1977 by American Association for Clinical Chemistry

High-performance liquid chromatography of pyruvic and alpha- ketoglutaric acids and its application to urine samples

JC Liao, NE Hoffman, JJ Barboriak and DA Roth

Pyruvic and alpha-ketoglutaric acids were quantitatively and rapidly converted to quinoxalones by a new method, to enhance their detectability in the ultraviolet. The quinoxalones were separated by reversed-phase chromatography with use of 10 micronm particles packed in a 30-cm (length) column. An aqueous solution of ammonium acetate and methanol was used for linear gradient elution. The urinary excretion of pyruvic and alpha-ketoglutaric acids by normal individuals, diabetic patients, and patients with renal dysfunction was determined.


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