Clinical Chemistry
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Clinical Chemistry 23: 813-815, 1977;
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Clinical Chemistry, Vol 23, 813-815, Copyright © 1977 by American Association for Clinical Chemistry

Solid-phase enzyme immunoassay for hepatitis B surface antigen

R Wei, GJ Knight, DH Zimmerman and HE Bond

A solid-phase enzyme immunoassay is described for measuring hepatitis B surface antigen in human serum or plasma. Immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. In the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. Results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specificity.




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Copyright © 1977 by the American Association for Clinical Chemistry.