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Clinical Chemistry 23: 882-884, 1977;
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Clinical Chemistry, Vol 23, 882-884, Copyright © 1977 by American Association for Clinical Chemistry

Cholesterol determination in high-density lipoproteins separated by three different methods

MF Lopes-Virella, P Stone, S Ellis and JA Colwell

We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+. Values so obtained correlate well with values obtained with the heparin-Mn2+ precipitation technique (r = 0.95, CV less than 5% in 66% of the subjects studied and between 5 and 10% in the remaining ones) or by ultracentrifugal separation (r = 0.82, CV less than 5% in 80% of the subjects studied and between 5 and 10% in the remaining ones). Our precipitation technique is more appropriate for routine clinical laboratory use.


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Z. A. Muna, K. Doudin, J. Songstad, R. J. Ulvik, and R. K. Berge
Tetradecylthioacetic Acid Inhibits the Oxidative Modification of Low Density Lipoprotein and 8-Hydroxydeoxyguanosine Formation In Vitro
Arterioscler Thromb Vasc Biol, November 1, 1997; 17(11): 3255 - 3262.
[Abstract] [Full Text]


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Clin. Chem.Home page
R. Johnson, P. McNutt, S. MacMahon, and R. Robson
Use of the Friedewald Formula to Estimate LDL-Cholesterol in Patients with Chronic Renal Failure on Dialysis
Clin. Chem., November 1, 1997; 43(11): 2183 - 2184.
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Arterioscler. Thromb. Vasc. Bio.Home page
C. H. Tuck, S. Holleran, and L. Berglund
Hormonal Regulation of Lipoprotein(a) Levels: Effects of Estrogen Replacement Therapy on Lipoprotein(a) and Acute Phase Reactants in Postmenopausal Women
Arterioscler Thromb Vasc Biol, September 1, 1997; 17(9): 1822 - 1829.
[Abstract] [Full Text]


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Clin. Chem.Home page
M. Nauck, W. Marz, J. Jarausch, C. Cobbaert, A. Sagers, D. Bernard, J. Delanghe, G. Honauer, P. Lehmann, E. Oestrich, et al.
Multicenter evaluation of a homogeneous assay for HDL-cholesterol without sample pretreatment
Clin. Chem., September 1, 1997; 43(9): 1622 - 1629.
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J. Clin. Endocrinol. Metab.Home page
R. Sipilainen, M. Uusitupa, S. Heikkinen, A. Rissanen, and M. Laakso
Variants in the Human Intestinal Fatty Acid Binding Protein 2 Gene in Obese Subjects
J. Clin. Endocrinol. Metab., August 1, 1997; 82(8): 2629 - 2632.
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Arterioscler. Thromb. Vasc. Bio.Home page
J. Jeppesen, H. O. Hein, P. Suadicani, and F. Gyntelberg
Relation of High TG–Low HDL Cholesterol and LDL Cholesterol to the Incidence of Ischemic Heart Disease: An 8-Year Follow-up in the Copenhagen Male Study
Arterioscler Thromb Vasc Biol, June 1, 1997; 17(6): 1114 - 1120.
[Abstract] [Full Text]


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Clin. Chem.Home page
Y.-C. Huang, J.-T. Kao, and K.-S. Tsai
Evaluation of two homogeneous methods for measuring high-density lipoprotein cholesterol
Clin. Chem., June 1, 1997; 43(6): 1048 - 1055.
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J. Clin. Endocrinol. Metab.Home page
B. Capaldo, L. Patti, U. Oliviero, S. Longobardi, F. Pardo, F. Vitale, S. Fazio, F. Di Rella, B. Biondi, G. Lombardi, et al.
Increased Arterial Intima-Media Thickness in Childhood-Onset Growth Hormone Deficiency
J. Clin. Endocrinol. Metab., May 1, 1997; 82(5): 1378 - 1381.
[Abstract] [Full Text] [PDF]


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Arterioscler. Thromb. Vasc. Bio.Home page
S. N. Pimstone, J. C. Defesche, S. M. Clee, H. D. Bakker, M. R. Hayden, and J. J.P. Kastelein
Differences in the Phenotype Between Children With Familial Defective Apolipoprotein B-100 and Familial Hypercholesterolemia
Arterioscler Thromb Vasc Biol, May 1, 1997; 17(5): 826 - 833.
[Abstract] [Full Text]


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Clin. Chem.Home page
N. Harris, V. Galpchian, J. Thomas, E. Iannotti, T. Law, and N. Rifai
Three generations of high-density lipoprotein cholesterol assays compared with ultracentrifugation/dextran sulfate–Mg2+ method
Clin. Chem., May 1, 1997; 43(5): 816 - 823.
[Abstract] [Full Text] [PDF]


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CLIN APPL THROMB HEMOSTHome page
M. Catalano, E. Perilli, G. Carzaniga, G. Scandale, and M. Carotta
Arterial Damage, Triglycerides, Apolipoprotein, and Lp-(a) Values in PVD Patients
Clinical and Applied Thrombosis/Hemostasis, April 1, 1997; 3(2): 104 - 109.
[Abstract] [PDF]


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Clin. Chem.Home page
P. N. M. Demacker, M. Hessels, H. Toenhake-Dijkstra, and H. Baadenhuijsen
Precipitation methods for high-density lipoprotein cholesterol measurement compared, and final evaluation under routine operating conditions of a method with a low sample-to-reagent ratio
Clin. Chem., April 1, 1997; 43(4): 663 - 668.
[Abstract] [Full Text] [PDF]


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Arterioscler. Thromb. Vasc. Bio.Home page
J. A. Kuivenhoven, P. de Knijff, J. M.A. Boer, H. A. Smalheer, G.-J. Botma, J. C. Seidell, J. J.P. Kastelein, and P. H. Pritchard
Heterogeneity at the CETP Gene Locus : Influence on Plasma CETP Concentrations and HDL Cholesterol Levels
Arterioscler Thromb Vasc Biol, March 1, 1997; 17(3): 560 - 568.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
J. A. Kuivenhoven, B. E. Groenemeyer, J. M. A. Boer, P. W. A. Reymer, R. Berghuis, T. Bruin, H. Jansen, J. C. Seidell, and J. J. P. Kastelein
Ser447stop Mutation in Lipoprotein Lipase Is Associated With Elevated HDL Cholesterol Levels in Normolipidemic Males
Arterioscler Thromb Vasc Biol, March 1, 1997; 17(3): 595 - 599.
[Abstract] [Full Text]


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J. Clin. Endocrinol. Metab.Home page
B. Imthurn, M. Rosselli, A. W. Jaeger, P. J. Keller, and R. K. Dubey
Differential Effects of Hormone-Replacement Therapy on Endogenous Nitric Oxide (Nitrite/Nitrate) Levels in Postmenopausal Women Substituted with 17{beta}-Estradiol Valerate and Cyproterone Acetate or Medroxyprogesterone Acetate
J. Clin. Endocrinol. Metab., February 1, 1997; 82(2): 388 - 394.
[Abstract] [Full Text] [PDF]


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Arterioscler. Thromb. Vasc. Bio.Home page
A. Broijersen, M. Eriksson, B. Leijd, B. Angelin, and P. Hjemdahl
No Influence of Simvastatin Treatment on Platelet Function In Vivo in Patients With Hypercholesterolemia
Arterioscler Thromb Vasc Biol, February 1, 1997; 17(2): 273 - 278.
[Abstract] [Full Text]


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Arch OphthalmolHome page
J. A. Mares-Perlman, R. Klein, B. E. K. Klein, J. L. Greger, W. E. Brady, M. Palta, and L. L. Ritter
Association of Zinc and Antioxidant Nutrients With Age-Related Maculopathy
Arch Ophthalmol, August 1, 1996; 114(8): 991 - 997.
[Abstract] [PDF]


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Arterioscler. Thromb. Vasc. Bio.Home page
A. Broijersen, M. Eriksson, B. Wiman, B. Angelin, and P. Hjemdahl
Gemfibrozil Treatment of Combined Hyperlipoproteinemia : No Improvement of Fibrinolysis Despite Marked Reduction of Plasma Triglyceride Levels
Arterioscler Thromb Vasc Biol, April 1, 1996; 16(4): 511 - 516.
[Abstract] [Full Text]


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BMJHome page
H. O. Hein, P. Suadicani, and F. Gyntelberg
Alcohol consumption, serum low density lipoprotein cholesterol concentration, and risk of ischaemic heart disease: six year follow up in the Copenhagen male study
BMJ, March 23, 1996; 312(7033): 736 - 741.
[Abstract] [Full Text]


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ANGIOLOGYHome page
M. Senti, J. Pedro-Botet, J. Rubies-Prat, and F. Vidal-Barraquer
Secondary Prevention of Coronary Heart Disease in Patients with Extracoronary Atherosclerosis: A Need for Accuracy of Low Density Lipoprotein Determination
Angiology, March 1, 1996; 47(3): 241 - 246.
[Abstract] [PDF]


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BMJHome page
T S Han, E M van Leer, J C Seidell, and M E J Lean
Waist circumference action levels in the identification of cardiovascular risk factors: prevalence study in a random sample
BMJ, November 25, 1995; 311(7017): 1401 - 1405.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
H. Olivecrona, A. G. Johansson, E. Lindh, S. Ljunghall, L. Berglund, and B. Angelin
Hormonal Regulation of Serum Lipoprotein(a) Levels : Contrasting Effects of Growth Hormone and Insulin-Like Growth Factor–I
Arterioscler Thromb Vasc Biol, July 1, 1995; 15(7): 847 - 849.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
H. M.G. Princen, W. van Duyvenvoorde, R. Buytenhek, A. van der Laarse, G. van Poppel, J. A. G. Leuven, and V. W.M. van Hinsbergh
Supplementation With Low Doses of Vitamin E Protects LDL From Lipid Peroxidation in Men and Women
Arterioscler Thromb Vasc Biol, March 1, 1995; 15(3): 325 - 333.
[Abstract] [Full Text]


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Arch OphthalmolHome page
R. Klein, B. E. K. Klein, and S. E. Moss
Age-Related Eye Disease and Survival: The Beaver Dam Eye Study
Arch Ophthalmol, March 1, 1995; 113(3): 333 - 339.
[Abstract] [PDF]


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Arterioscler. Thromb. Vasc. Bio.Home page
A. Broijersen, M. Eriksson, B. Angelin, and P. Hjemdahl
Gemfibrozil Enhances Platelet Activity in Patients With Combined Hyperlipoproteinemia
Arterioscler Thromb Vasc Biol, January 1, 1995; 15(1): 121 - 127.
[Abstract] [Full Text]


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Arch Pediatr Adolesc MedHome page
M. Panamonta, N. Settasatian, E. L. Kaplan, and A. Chaikitpinyo
Serum Cholesterol Levels in Patients With Acute Rheumatic Fever
Arch Pediatr Adolesc Med, July 1, 1993; 147(7): 732 - 736.
[Abstract] [PDF]


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Hum Exp ToxicolHome page
A.A. Abdel-Gayoum, B.H. Ali, K. Ghawarsha, and A.A. Bashir
Plasma Lipid Profile in Rats with Gentamicin-Induced Nephrotoxicity
Human and Experimental Toxicology, January 1, 1993; 12(5): 371 - 375.
[Abstract] [PDF]


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Arch Intern MedHome page
R. A. Reinhart, K. Gani, M. R. Arndt, and S. K Broste
Apolipoproteins A-I and B as Predictors of Angiographically Defined Coronary Artery Disease
Arch Intern Med, August 1, 1990; 150(8): 1629 - 1633.
[Abstract] [PDF]


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Arch Intern MedHome page
E. S. K. Choi, R. B. McGandy, G. E. Dallal, R. M. Russell, R. A. Jacob, E. J. Schaefer, and J. A. Sadowski
The Prevalence of Cardiovascular Risk Factors Among Elderly Chinese Americans
Arch Intern Med, February 1, 1990; 150(2): 413 - 418.
[Abstract] [PDF]


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ANGIOLOGYHome page
K. Prasad and J. Kalra
Experimental Atherosclerosis and Oxygen Free Radicals
Angiology, September 1, 1989; 40(9): 835 - 843.
[Abstract] [PDF]


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JPEN J Parenter Enteral NutrHome page
U. Julius and W. Leonhardt
Elimination and Metabolism of a Fat Emulsion Containing Medium Chain Triglycerides (Lipofundin MCT 10%)
JPEN J Parenter Enteral Nutr, March 1, 1988; 12(2): 116 - 120.
[Abstract] [PDF]




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