Clinical Chemistry
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Clinical Chemistry 23: 1281-1283, 1977;
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Clinical Chemistry, Vol 23, 1281-1283, Copyright © 1977 by American Association for Clinical Chemistry

Determination of "true" serum creatinine by high-performance liquid chromatography combined with a continuous-flow microanalyzer

ND Brown, HC Sing, WE Neeley and SE Koetitz

We coupled a high-performance liquid chromatograph with a continuous- flow microanalyzer to produce a system for specifically determinig "true" creatinine in urine and serum specimens. A selective microbore pellicular cation-exchange column and a single eluting sodium citrate buffer are used to separate the noncreatinine but Jaffe-positive constituents from the creatinine in normal and experimental specimens. The effluent is analyzed continuously, on-line, the alkaline picrate complex being developed and measured in the microanalyzer. Physiological samples, reference standards, and internal control specimens are assayed in 6-min intervals subsequent to the initial injection. The relationship between concentration and peak are is linear for creatinine standards between 5 and 10 mg/liter. Specimen volumes ranging from 1 to 25 microliters, and containing creatinine in amounts exceeding 5 ng per injected sample, can be assayed with this system.


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G. L. Myers, W. G. Miller, J. Coresh, J. Fleming, N. Greenberg, T. Greene, T. Hostetter, A. S. Levey, M. Panteghini, M. Welch, et al.
Recommendations for Improving Serum Creatinine Measurement: A Report from the Laboratory Working Group of the National Kidney Disease Education Program
Clin. Chem., January 1, 2006; 52(1): 5 - 18.
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Copyright © 1977 by the American Association for Clinical Chemistry.