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Clinical Chemistry 23: 1306-1309, 1977;
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Clinical Chemistry, Vol 23, 1306-1309, Copyright © 1977 by American Association for Clinical Chemistry

Measurement of thiamylal and thiopental in plasma by electron capture and flame photometric gas-liquid chromatography

RH Smith, JA MacDonald, DS Thompson and WE Flacke

We describe an isothermal gas-liquid chromatographic procedure developed for measuring thiamylal and thiopental in plasma. The unchanged drug is extracted into ethyl acetate from acidified plasma, together with an internal standard. The solvent is removed, the residue methylated, aliquots, diluted with benzene, are injected into a 183-cm gas-liquid chromatographic column containing 3% OV-17. Sensitivity of detection is in the nanogram to picogram range.




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Copyright © 1977 by the American Association for Clinical Chemistry.