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Clinical Chemistry 23: 1321-1323, 1977;
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Clinical Chemistry, Vol 23, 1321-1323, Copyright © 1977 by American Association for Clinical Chemistry

Improved colorimetric determination of serum zinc

DJ Johnson, YY Djuh, J Bruton and HL Williams

We show how zinc may easily be quantified in serum by first using an optimum concentration of guanidine hydrochloride to cause release of zinc from proteins, followed by complexation of released metals with cyanide. The cyanide complex of zinc is preferentially demasked with chloral hydrate, followed by a colorimetric reaction between zinc and 4- (2-pyridylazo)resorcinol. This is a sensitive water-soluble ligand; its complex with zinc has an absorption maximum at 497 nm. Values found by this technique compare favorably with those obtained by atomic absorption spectroscopy.




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Copyright © 1977 by the American Association for Clinical Chemistry.