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Clinical Chemistry 23: 1335-1337, 1977;
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Clinical Chemistry, Vol 23, 1335-1337, Copyright © 1977 by American Association for Clinical Chemistry

Separation of hydroxyproline-containing protein from C1q (a subcomponent of complement) in serum

CL Rosano and C Hurwitz

Precipitation of the euglobulin fraction from serum separates C1q from another hydroxyproline-containing protein(s), which remains in the serum-minus-euglobulin fraction. The presence of C1q in the euglobulin fraction has been verified by radial immunodiffusion assay, by sensitivity to collagenase, by hydroxyproline content, and by electrofocusing. The presence of another hydroxyproline-containing protein(s) in the serum-minus-euglobulin fraction has been verified by the same criteria. The isoelectric pH range of C1q is 6.1-7.0; the isoelectric pH range of the other hydroxyproline-containing protein(s) is 4.2-5.5.





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Copyright © 1977 by the American Association for Clinical Chemistry.