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Clinical Chemistry 23: 1338-1339, 1977;
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Clinical Chemistry, Vol 23, 1338-1339, Copyright © 1977 by American Association for Clinical Chemistry

Micromethod for determination of diazepam by electron-capture gas- liquid chromatography

E Rey, JM Turquais and G Olive

We used 100 microliters of plasma for the determination of diazepam. After the internal standard, prazepam, is added, the serum is directly extracted with diethyl ether, the extract is evaporated, the residue is dissolved in ethanol, and the drug is measured by gas-liquid chromatography, with use of an electron capture detector. With this procedure, 2.5 ng of diazepam in the sample can be speedily measured with specificity, accuracy, and reproducibility (CV = 4.5%).




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Copyright © 1977 by the American Association for Clinical Chemistry.