Clinical Chemistry
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Clinical Chemistry 23: 1527-1530, 1977;
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Clinical Chemistry, Vol 23, 1527-1530, Copyright © 1977 by American Association for Clinical Chemistry

Some observations on the kinetics of the Jaffe reaction for creatinine

RM Shoucri and M Pouliot

The Jaffe reaction for creatinine assay appears to follow pseudo-first- order kinetics; first-order rate constants are different for different samples. Rate constants for 10 different serum samples varied from a low value of 0.0040 +/- 0.0003 s-1 to 0.0084 +/- 0.0008 s-1. We describe an approach for determining first-order rate constants from kinetic data and discuss the effects of the above observations on the mathematical formulations required for reliable kinetic determinations of creatinine.




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Copyright © 1977 by the American Association for Clinical Chemistry.