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Clinical Chemistry 23: 1538-1540, 1977;
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Clinical Chemistry, Vol 23, 1538-1540, Copyright © 1977 by American Association for Clinical Chemistry

Enzyme-coupled measurement of uric acid in serum with a centrifugal analyzer

RM White, RE Cross and J Savory

An early-reading blank-corrected end-point determination of uric acid in serum has been developed for use with a centrifugal analyzer. The method is based on a modification of the uricase (urate:oxygen oxidoreductase, EC 1.7.3.3)/catalase (hydrogen peroxide:hydrogen peroxide oxidoreductase EC 1.11.1.6)/aldehyde dehydrogenase (aldehyde:NAD(P)+ oxidoreductase, EC 1.2.1.5)-coupled analytical scheme reported by Haeckel [Z. Klin. Chem. Klin. Biochem. 14, 101 (1976)]. Sensitivity and precision of the method are excellent, and results compare well with those obtained by the Kageyama procedure [Clin. Chim. Acta 31, 421 (1971)].




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Copyright © 1977 by the American Association for Clinical Chemistry.