Clinical Chemistry
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Clinical Chemistry 23: 1599-1601, 1977;
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Clinical Chemistry, Vol 23, 1599-1601, Copyright © 1977 by American Association for Clinical Chemistry

Lysozyme determined in serum or urine by a simple nephelometric method

J Borgen and I Romslo

We describe a nephelometric procedure for determination of lysozyme. A quantitative result is obtained in 2 to 3 min with 50 microliter of serum or urine. At a lysozyme concentration of 12.0 mg/liter, between- day precision was 3.4% and the analytical recovery 96.5-105.5%. The values obtained were a linear function of enzyme concentration below 50 mg/liter, and they agreed with those obtained by absorptiometric determination of the decrease in turbidity of a suspension of Micrococcus lysodeikticus.




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Copyright © 1977 by the American Association for Clinical Chemistry.